An attempt to improve the immunization of sheep against heart-water by using different combinations of 3 stocks of Cowdria ruminantium.
نویسندگان
چکیده
Neither sheep immune to the Ball 3, the Kwanyanga or the Mara stocks of Cowdria ruminantium nor those immunized with combinations of these 3 stocks were protected against challenge with the Mali stock. Against challenge with the Welgevonden stock, however, immunization with each of the 3 combinations and with single stocks effected a protective immunity.
منابع مشابه
Vaccination against heartwater using in vitro attenuated Cowdria ruminantium organisms.
Sequential passage of Cowdria ruminantium (Senegal isolate) in cultures of bovine umbilical endothelial cells has resulted in loss of virulence without loss of immunogenicity, as previously demonstrated. We have carried out further immunization of 39 Dutch sheep using in vitro attenuated rickettsiae of passage 21 and challenged these animals either with the homologous or with heterologous Cowdr...
متن کاملIdentification of the antigenic proteins of Cowdria ruminantium.
Immunoblotting of Cowdria ruminantium proteins with sheep or bovine antiserum identified 2 antigenically conserved proteins, one being an immunodominant 31 kDa and the other a minor 27 kDa protein. These proteins are present in the electrophoretic profiles of the Welgevonden, Ball 3 and Kwanyanga stocks and are recognized by sheep antiserum to the Welgevonden, Ball 3, Kwanyanga, Mali, Comoro, B...
متن کاملIncreased pathogenicity of an Ehrlichia-like agent after passage through Amblyomma hebraeum: a preliminary report.
After being passaged through 3 generations of Amblyomma hebraeum, an Ehrlichia-like agent isolated from an adult Hyalomma truncatum female became more pathogenic and elicited a disease in sheep indistinguishable from heartwater. Cross-immunity between this agent and several stocks of Cowdria ruminantium and high levels of antibody elicited by the agent against 2 stocks of C. ruminantium in the ...
متن کاملThe heterogenicity of Cowdria ruminantium stocks: cross-immunity and serology in sheep and pathogenicity to mice.
Ten stocks of Cowdria ruminantium (Ball 3, Breed, Comoro, Germishuys, Kümm, Kwanyanga, Mali, Mara, Nonile and Welgevonden) were compared from a cross-immunity, serological and mouse pathogenicity point of view. They were found to differ in varying degrees. Except for the Ball 3, Comoro and Germishuys stocks that were similar but not identical, there was no pattern in the antigenic diversity of ...
متن کاملIdentification of an immunodominant antigenically conserved 32-kilodalton protein from Cowdria ruminantium.
Western blotting (immunoblotting) of Cowdria ruminantium antigens with goat or mouse antiserum identified a periodate-resistant, proteinase K-sensitive immunodominant antigen of 32,000 daltons. This protein, designated Cr32, could be demonstrated in goat choroid plexus infected with one of two different Cowdria stocks. Antisera against nine different Cowdria stocks from Africa and the Caribbean...
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 57 3 شماره
صفحات -
تاریخ انتشار 1990